α1 integrin subunit  (R&D Systems)

Journal: International journal of molecular sciences

Article Title: Oleuropein Stimulates Migration of Human Trophoblast Cells and Expression of Invasion-Associated Markers.

doi: 10.3390/ijms25010500

Figure Lengend Snippet: Figure 5. Effect of oleuropein (OLE) on the expression of integrin subunits α1 (ITGα1) (A) and β1 (ITGβ1) (B) in HTR-8/SVneo cells at the protein level, using the CELISA method. * p < 0.05.

Article Snippet: After blocking, 50 μL of each primary antibody for α1 integrin subunit (MAB5676, source: mouse, 1:400, R&D Systems, MN, USA) or β1 integrin subunit (AB1952, source: rabbit, 1:500, Millipore Sigma, Burlington, MA, USA) or COX-2 (PA5-27238, source: rabbit, 1:500, Invitrogen, Waltham, MA, USA) was added in PBS with 1% BSA to the wells and incubated overnight at 4 ◦C in a humidified chamber.

Techniques: Expressing

Journal: Brain research

Article Title: Chronic mild hypoxia increases expression of laminins 111 and 411 and the laminin receptor α6β1 integrin at the blood-brain barrier

doi: 10.1016/j.brainres.2018.07.012

Figure Lengend Snippet: Characterization of laminin receptor expression by cerebral blood vessels. Dual-IF was performed on frozen brain sections using antibodies specific for CD31 (AlexaFluor-488) and antibodies specific for the integrin subunits α1, α6, or β1 or the β-dystroglycan subunit. Scale bar = 100 μm. Note that cerebral blood vessels stain positive for the αl, α6, and β1 integrin subunits as well as for β-dystroglycan.

Article Snippet: The following antibodies were used in this study: rat monoclonal antibodies reactive for CD31 (clone MEC13.3) and the integrin subunits α6 (clone GoH3) and β1 (clone 9EG7) the hamster monoclonal reactive for the integrin α1 subunit (clone Ha31/8) (all from BD Pharmingen, La Jolla, CA), hamster anti-CD31 (clone 2H8) from Abcam, Cambridge, MA, mouse anti-β-dystroglycan from Novocastra, Newcastle upon-Tyne, England, and rabbit anti-laminin from Sigma, St. Louis, MO.

Techniques: Expressing, Staining

Journal: Brain research

Article Title: Chronic mild hypoxia increases expression of laminins 111 and 411 and the laminin receptor α6β1 integrin at the blood-brain barrier

doi: 10.1016/j.brainres.2018.07.012

Figure Lengend Snippet: Chronic mild hypoxia (CMH) specifically upregulates α6β1 integrin expression in cerebral blood vessels. A. Frozen sections of brain taken from mice exposed to normoxia or 2, 4, 7, or 14 days CMH (8% O2) were stained using antibodies specific for the integrin subunits α1,α6,or β1 or the β-dystroglycan subunit. Scale bar =100 μm. B -E. Quantification of cerebrovascular expression of the integrin subunits αl (B), α6 (C), β1 (D) or β-dystroglycan (E) at different time-points of CMH. Results are expressed as the mean ± SEM (n = 4 mice/group). Note that cerebrovascular expression levels of the α6 and β1 integrin subunits were significantly increased after 4 and 7 days CMH. ** p < 0.01 vs. normoxia (0 days hypoxia) and vs. 2 days hypoxia. * p < 0.05 vs. 4 days hypoxia.

Article Snippet: The following antibodies were used in this study: rat monoclonal antibodies reactive for CD31 (clone MEC13.3) and the integrin subunits α6 (clone GoH3) and β1 (clone 9EG7) the hamster monoclonal reactive for the integrin α1 subunit (clone Ha31/8) (all from BD Pharmingen, La Jolla, CA), hamster anti-CD31 (clone 2H8) from Abcam, Cambridge, MA, mouse anti-β-dystroglycan from Novocastra, Newcastle upon-Tyne, England, and rabbit anti-laminin from Sigma, St. Louis, MO.

Techniques: Expressing, Staining